Microorganism |
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Enterobacter cloacae strain CF-S27 |
Taxonomy |
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Electron Acceptor |
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Oxygen; Acetamide |
Electron Donor |
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Ammonium heptamolybdate |
Information about Article |
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Reference:Padhi et al., 2017 Title:Aerobic and heterotrophic nitrogen removal by Enterobacter cloacae CF-S27 with efficient utilization of hydroxylamine Pubmed ID:28242385.0 Pubmed link:Link Full research link:Link Abstract: Heterotrophic bacterium, Enterobacter cloacae CF-S27 exhibited simultaneous nitrification and aerobic denitrification in presence of high concentration of hydroxylamine. With the initial nitrogen concentration of 100 mg L−1 h−1, ammonium, nitrate and nitrite removal efficiencies were 81%, 99.9% and 92.8%, while the corresponding maximum removal rates reached as high as 11.6, 15.1 and 11.2 mg L−1 h−1 respectively. Quantitative amplification by real time PCR and enzyme assay demonstrated that hydroxylamine reductase gene (hao) is actively involved in hetrotrophic nitrification and aerobic denitrification process of Enterobacter cloacae CF-S27. PCR primers were designed targeting amplification of hao gene from diversified environmental soil DNA. The strain Enterobacter cloacae CF-S27 significantly maintained the undetectable amount of dissolved nitrogen throughout 60 days of zero water exchange fish culture experiment in domestic wastewater. |