Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Detailed information

Microorganism

Enterobacter cloacae strain CF-S27

Taxonomy

  • Phylum : Proteobacteria
  • Class : Gammaproteobacteria
  • Order :Enterobacterales
  • Family : Enterobacteriaceae
  • Genus : Enterobacter

Electron Acceptor

Oxygen; Acetamide

Electron Donor

Ammonium heptamolybdate

Information about Article

Reference:Padhi et al., 2017

Title:Aerobic and heterotrophic nitrogen removal by Enterobacter cloacae CF-S27 with efficient utilization of hydroxylamine

Pubmed ID:28242385.0

Pubmed link:Link

Full research link:Link

Abstract: Heterotrophic bacterium, Enterobacter cloacae CF-S27 exhibited simultaneous nitrification and aerobic denitrification in presence of high concentration of hydroxylamine. With the initial nitrogen concentration of 100 mg L−1 h−1, ammonium, nitrate and nitrite removal efficiencies were 81%, 99.9% and 92.8%, while the corresponding maximum removal rates reached as high as 11.6, 15.1 and 11.2 mg L−1 h−1 respectively. Quantitative amplification by real time PCR and enzyme assay demonstrated that hydroxylamine reductase gene (hao) is actively involved in hetrotrophic nitrification and aerobic denitrification process of Enterobacter cloacae CF-S27. PCR primers were designed targeting amplification of hao gene from diversified environmental soil DNA. The strain Enterobacter cloacae CF-S27 significantly maintained the undetectable amount of dissolved nitrogen throughout 60 days of zero water exchange fish culture experiment in domestic wastewater.